1. Product Overview & Research Grade Applications

Ketamine Hydrochloride (C13H17Cl2NO\text{C}_{13}\text{H}_{17}\text{Cl}_2\text{NO}C13​H17​Cl2​NO) is an arylcyclohexylamine compound widely recognized for its diverse pharmacological activities. Manufactured under rigorous quality control standards at Aura Biochemicals, our 250mg analytical-grade Ketamine Hydrochloride reference standard is processed to meet the demanding requirements of analytical laboratories, academic research, and pharmaceutical formulation testing.

Primary Laboratory Applications

• Analytical Reference Standard: Used for qualitative and quantitative analysis in High-Performance Liquid Chromatography (HPLC), Gas Chromatography-Mass Spectrometry (GC-MS), and Nuclear Magnetic Resonance (NMR) spectroscopy.

• Neuropharmacological Research: Serves as a key ligand in studying non-competitive antagonism of the N-methyl-D-aspartate (NMDA) receptor complex.

• Depression & Synaptogenesis Research: Crucial for investigating rapid-acting antidepressant pathways, glutamatergic neurotransmission, and dendritic spine density changes in animal models.

• Analgesic Mechanism Mapping: Utilized in preclinical studies mapping central nervous system pain signaling, opioid-sparing mechanisms, and chronic pain pathways.

2. Chemical Identification & Structural Properties

Ketamine Hydrochloride is chemically designated as $(\pm )-2-(2\text{-chlorophenyl})-2\text{-(methylamino)cyclohexan-1-one hydrochloride}$. It exists as a racemic mixture of two enantiomers: $(R)\text{-ketamine}$ and $(S)\text{-ketamine}$.

Molecular Structure & Characteristics

• IUPAC Name: 2-(2-chlorophenyl)-2-(methylamino)cyclohexan-1-one hydrochloride

• Canonical SMILES: CN1CCCCC1(=O)C2=CC=CC=C2Cl.Cl

• InChI Key: YTFGGWJGKSLKNG-UHFFFAOYSA-N

• Monoisotopic Mass: $237.092\text{ g/mol}$ (Free Base)

• pH Range: $3.5-5.5$ ($10\%$ aqueous solution)

3. Pharmacological & Biochemical Mechanisms

Understanding the mechanism of action of Ketamine Hydrochloride is essential for research applications in neurobiology and psychopharmacology:

1. NMDA Receptor Blockade: Ketamine interacts directly with the phencyclidine (PCP) binding site inside the NMDA receptor ion channel, inhibiting calcium inflow and down-regulating downstream signaling cascades.

2. AMPA Receptor Activation: By inhibiting NMDA receptors on GABAergic interneurons, Ketamine induces a glutamate burst, leading to the activation of post-synaptic $\alpha$-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors.

3. mTORC1 Pathway Induction: AMPA receptor depolarization triggers brain-derived neurotrophic factor (BDNF) release, activating mammalian target of rapamycin complex 1 (mTORC1) and promoting synaptogenesis.

4. Monoaminergic Interactions: Interacts with opioid receptors ($\mu$ and $\kappa$), monoamine transporters (norepinephrine, dopamine, serotonin), and muscarinic cholinergic receptors.

4. Quality Assurance & Analytical Certification

Every 250mg lot of Ketamine Hydrochloride supplied by Aura Biochemicals undergoes stringent testing to ensure reproducibility across experimental trials.

Certificates of Analysis (CoA) Included Per Batch:

• HPLC Chromatogram: Confirms peak purity, retention time match, and absence of significant process impurities.

• Mass Spectrum (MS): Verifies the exact molecular ion mass ($m/z$).

• Fourier-Transform Infrared (FT-IR) Spectroscopy: Confirms structural identity against established standard libraries.

• Heavy Metals & Residual Solvent Testing: Ensures compliance with ICH Q3A/B/C guidelines.

5. Storage, Handling & Safety Protocols

Safety Warning: Ketamine Hydrochloride 250mg is intended strictly for qualified research, analytical standard, and industrial manufacturing purposes. Not for direct human or veterinary administration without applicable regulatory clearances.

Handling Guidelines

• Personal Protective Equipment (PPE): Handle in a certified chemical fume hood or cleanroom environment. Wear safety goggles, chemical-resistant nitrile gloves, and a lab coat.

• Storage Requirements: Keep container tightly sealed in a cool, dry, and well-ventilated area (15∘C−25∘C15^\circ\text{C} – 25^\circ\text{C}15∘C−25∘C). Protect from direct sunlight and humidity.

• Spill Response: Neutralize dust generation. Sweep up carefully into an appropriate waste container for chemical disposal according to local environmental regulations.

6. Ordering, Compliance & Logistics

Aura Biochemicals operates under strict compliance with regional and international chemical distribution regulations.

• Documentation Requirements: Procurement of Ketamine Hydrochloride 250mg requires verified institutional credentials, authorized license verification, and End-User Declarations (EUD) where applicable.

• Packaging: Sealed in tamper-evident, light-resistant glass vials within secondary protective containment.

• Shipping & Delivery: Shipped with temperature tracking and compliant hazardous/controlled material tracking labels across authorized global destinations.

Frequently Asked Questions (FAQ)

1. What is the purity level of Aura Biochemicals’ Ketamine Hydrochloride 250mg?

Our product is purified to $\ge 99.5\%$ (HPLC grade) with a detailed Certificate of Analysis (CoA) provided for every individual batch.

2. Is this product supplied as a free base or a salt?

This product is supplied as the hydrochloride salt form ($\text{Ketamine HCl}$), offering high water solubility and superior chemical stability compared to the free base.

3. How can I obtain a Safety Data Sheet (SDS) and Certificate of Analysis (CoA)?

CoAs are shipped directly with your order. Digital copies of both the SDS and batch-specific CoA can be requested directly through the Aura Biochemicals Customer Portal or by contacting our technical support team.

Request a Quote or Order Technical Samples

For bulk procurement inquiries, custom packaging formats, or institutional verification, contact our sales support at sales@aurabiochemicals.com or submit an inquiry through the Aura Biochemicals web portal.

Bulk Procurement, Institutional Supply & Commercial Ordering

Search-Optimized Procurement Guide for Ketamine HCl 250mg

When sourcing high-purity Ketamine Hydrochloride 250mg (CAS 1867-66-9) for quantitative assay calibration, clinical assay development, or preclinical neurobiology research, chemical consistency and regulatory transparency are critical. Aura Biochemicals delivers certified, ultra-pure analytical standards manufactured to exceed strict USP, EP, and ISO testing specifications.

       [ Procurement Workflow: Aura Biochemicals ]
       
  1. Submit Verification    2. Obtain Custom Quote   3. Express Compliant Shipping
+------------------------+  +---------------------+  +--------------------------+
| • License Verification |->| • Tiered Pricing    |->| • Cold-Chain / Sealed    |
| • EUD Form Processing  |  | • CoA & SDS Package |  | • Traceable Delivery     |
+------------------------+  +---------------------+  +--------------------------+

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Tiered Institutional Pricing & Batch Volume Scale

We offer scalable volume discounts for accredited CROs, university chemistry departments, diagnostic laboratories, and pharmaceutical R&D facilities.

Why Procurement Teams Choose Aura Biochemicals

• Batch-to-Batch Reproducibility: Every lot of Ketamine Hydrochloride is verified by dual-method testing (HPLC + FT-IR) to ensure consistent analytical baseline readings across multi-year trials.

• Compliant Chain of Custody: We handle all controlled substance documentation, End-User Declarations (EUD), and export authorization checks to eliminate delivery delays.

• Tamper-Evident Ultra-Pure Packaging: Packaged in borosilicate glass vials sealed under inert argon gas to prevent moisture absorption and ambient atmospheric oxidation.

• Turnkey Documentation Package: Every order ships with a physical Certificate of Analysis (CoA), Safety Data Sheet (SDS), and exact isotopic / structural mass spectra validation.

Streamlined Order Placement Process

Instant Procurement Assistance

Need custom packaging, special solution formulation ($1.0 \text{ mg/mL}$ in methanol), or a bulk quotation for Ketamine Hydrochloride 250mg?

• Sales Email: sales@aurabiochemicals.com

• Direct Order Line: Emails Us for Our Contact number

• Secure Portal: Access the Aura Biochemicals Direct Order Portal to request instant quotes and download batch-specific CoAs.